Rapid Microdilution-Colorimetric Assay for Yeast Susceptibility to Fluorocytosine

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Rapid microdilution-colorimetric assay for yeast susceptibility to fluorocytosine.

Acid production by certain yeast species through the fermentation of glucose was used as the basis of an in vitro test for measuring susceptibility of these organisms to 5-fluorocytosine. Serial dilutions of 5-fluorocytosine in yeast nitrogen base broth, with bromothymol blue indicator dye, were made on microtiter plates. A fixed-concentration suspension of yeast cells was added to successive w...

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ژورنال

عنوان ژورنال: Antimicrobial Agents and Chemotherapy

سال: 1977

ISSN: 0066-4804,1098-6596

DOI: 10.1128/aac.12.5.614